ISSN: 2251-8363    eISSN: 2251-8819  
J Nephropathol. 2015;4(3):69-76.
doi:10.12860/jnp.2015.14
PMID: 26310144
PMCID: PMC4544557

Scopus id: 84936930562

Original Article

AT1R A1166C variants in patients with type 2 diabetes mellitus and diabetic nephropathy

Mahmoudreza Moradi 1, Zohreh Rahimi 2,3 * , Sonia Amiri 4, Ziba Rahimi 2, Mahmood Vessal 4, Hamid Nasri 5

1 Department of Urology and Regenerative Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Department of Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran
4 Department of Biochemistry, Fars Science and Research Branch, Islamic Azad University, Fars, Iran
5 Department of Nephrology, Isfahan University of Medical Sciences, Isfahan, Iran
*Corresponding author: Prof. Zohreh Rahimi, Medical Biology Research Center, Medical School, Kermanshah, Iran. Email: zrahimi@kums.ac.ir

Abstract

Background: There are inconsistent reports related to the role of angiotensin II type 1 receptor (AT1R) on the risk of type 2 diabetes mellitus (T2DM) and its renal complications.

Objectives: To identify the association between AT1R A1166C variants with the risk of T2DM and also with diabetic nephropathy (DN). Patients and Methods: In a case-control study, the AT1R A1166C polymorphism was detected in 135 T2DM patients with and without DN and in 98 healthy subjects from Western Iran. The genotypes of AT1R A1166C polymorphism were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.

Results: The frequencies of AT1R A1166C genotypes and alleles were not significantly difference between patients with and without DN and controls. The frequencies of rare allele of 1166 C were 10%, 16.5%, 15.9% and 15.3% in micro-, macro- and normo-albuminuric patients and in healthy individuals, respectively (P > 0.05). The systolic blood pressure and serum creatinine level in DN patients were significantly higher in carriers of AT1R CC compared to carriers of AT1R AA genotype. In the presence of uncontrolled hyperglycemia (HbA1c > 7.5%), there was a trend toward increased risk of macro-albuminuria in carriers of AC+CC genotype (OR=3.66, [95% CI: 0.81-16.58], P = 0.092).

Conclusions: Our study indicated the absence of an association between AT1R A1166C polymorphism with the risk of T2DM and DN. It seems in carriers of AT1R C allele systolic blood pressure and serum creatinine level to be higher compared to the A allele carriers.

Implication for health policy/practice/research/medical education:

The AT1R variants were not directly associated with the risk of T2DM or DN. However, the AT1R AC+CC genotype tended to increase the risk of macro-albuminuria in the presence of HbA1c >7.5%. Also, in carriers of AT1R 1166 C allele the systolic blood pressure and serum creatinine level were higher compared to the 1166 A allele carriers.

Please cite this paper as: Moradi M, Rahimi Z, Amiri S, Rahimi Z, Vessal M, Nasri H. AT1R A1166C variants in patients with type 2 diabetes mellitus and diabetic nephropathy. J Nephropathol. 2015;4(3):69-76. DOI: 10.12860/jnp.2015.14

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