ISSN: 2251-8363    eISSN: 2251-8819  
J Nephropathol. 2018;7(2):88-92.
doi:10.15171/jnp.2018.21

Original Article

The protective roles of zinc and estradiol in renal ischemia/ reperfusion injury in ovariectomized rats

Foroogh Barekat 1, Ardeshir Talebi 1, Mehdi Nematbakhsh *

1 Water and Electrolytes Research Center/Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran
2 IsfahanMN Institute of Basic and Applied Sciences Research, Isfahan, Iran
*Corresponding author: Prof. Mehdi Nematbakhsh, Ph.D; Email: nematbakhsh@med.mui.ac.ir

Abstract

Context: One of the main events associated with ischemia/reperfusion injury (IRI) is excessive production of reactive oxygen species (ROS). Zinc (Zn) and estradiol are considered as antioxidants that scavenge free radicals.

Objectives: The aim of this study was to compare the protective effect of Zn and estradiol against renal IRI in ovariectomized rats.

Materials and Methods: Ovariectomized Wistar rats were randomly divided into five experimental groups including control (sham operated), IRI, estradiol treated +IRI, estradiol and Zn treated +IRI, Zn treated +IRI groups. The IRI was induced by clamping renal vessels for 45 minutes followed by 24 hours reperfusion. During the last 6 hours of reperfusion, urine output was collected and the measurements were performed.

Results: IRI caused an increase in kidney tissue damage score (KTDS) significantly (P<0.05). The serum levels of blood urea nitrogen (BUN) and the creatinine (Cr) also elevated by IRI, but theses parameters attenuated by Zn treatment significantly (P<0.05). Cr-clearance and urine flow were increased by Zn, and the percent of sodium excretion was increased by estradiol significantly (P<0.05). The kidney tissue level of malondialdehyde was decreased by co-treatment of Zn and estradiol statistically (P<0.05).

Conclusions: Zn protected the kidney against IRI with an evident improvements of serum BUN and Cr levels, Cr clearance and tissue damage.

Key words: Zinc, Rat, Ischemia-rep, Estradiol

Implication for health policy/practice/research/medical education:

Administration of zinc (Zn) as an antioxidant agent demonstrated an efficient role in preventing renal dysfunction induced by renal ischemia/reperfusion injury (IRI) in female rats. It seems the overproduction of reactive oxygen species (ROS) during renal IRI may inhibit by Zn; however more studies may clarify the exact mechanism.

Please cite this paper as: Barekat F, Talebi A, Nematbakhsh M. The protective roles of zinc and estradiol in renal ischemia/ reperfusion injury in ovariectomized rats. J Nephropathol. 2018;7(2):88-92. DOI: 10.15171/jnp.2018.21.

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Submitted: 09 Jun 2017

Accepted: 20 Sep 2017
First published online: 25 Sep 2017
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