J Nephropathol. 2014;3(4):133-138.
doi: 10.12860/jnp.2014.26
PMID: 25374882
PMCID: PMC4219615
Scopus id: 84920940526
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Original Article

Effect of pomegranate flower extract on cisplatin-induced nephrotoxicity in rats

Fatemeh Motamedi 1,2, Mehdi Nematbakhsh 1,3,4 * , Ramesh Monajemi 2, Zahra Pezeshki 1, Ardeshir Talebi 1,5, Behzad Zolfaghari 1,6, Azam Mansoori 1, Shadan Saberi 1, Aghdas Dehghani 1, Farzaneh Ashrafi 1,7

1 Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Biology, Falavarjan Branch Islamic Azad University, Isfahan, Iran
3 Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran
4 Institute of Basic & Applied Sciences Research, Isfahan, Iran
5 Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan, Iran
6 Department of Pharmacognosy, Isfahan University of Medical Sciences, Isfahan, Iran
7 Department of Internal Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
*Corresponding author: Prof. Mehdi Nematbakhsh, Water & Electrolytes Research Center/Kidney Diseases Research Center/ Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran. Email: nematbakhsh@med.mui.ac.ir

Abstract

Background: Chemotherapy with cisplatin (CP) is accompanied with nephrotoxicity.

Objectives: In the current study, pomegranate flower extract (PFE) has been evaluated as an antioxidant agent against CP-induced-renal toxicity.

Materials and Methods: Thirty two male Wistar rats were divided into five groups (6-8 in each group). The animals in groups 1 to 3 received PFE (25 mg/kg), PFE (50 mg/kg), and placebo (saline), respectively for 9 days, and onset of the day 3, they also received CP (2.5 mg/kg/ day). Groups 4 and 5 were treated with PFE (25 and 50 mg/kg/day) for 9 days. Finally, the animals were sacrificed at day 9 after collecting blood samples. Kidneys were removed, weighted, and underwent histopathological investigation.

Results: The mean serum level of creatinine in group 3 (treated with CP and placebo) increased significantly (p<0.05), but the value decreased significantly (p<0.05) in group 1. Kidney weight in group 1 was lower than KW in groups 2 and 3, however it was significant when compared with group 2 (p<0.05). The serum nitrite level in group 2 was non-significantly lower than that in other groups, and no significant changes were observed in serum levels of malondialdehyde (MDA). Tissue level of nitrite was significantly decreased in the positive control and high dose of PFE plus CP-treated groups (p<0.05). Among CP-treated groups, low dose of PFE significantly improved kidney nitrite level (p<0.05). The results from histopathological staining indicated less tissue damage in group 1 when compared with group 3.

Conclusions: It seems that low dose of PFE plays a protective role against CP-induced renal toxicity in rats.

Implication for health policy/practice/research/medical education:

The low dose of pomegranate flower extract (25 mg/kg) provided protective effects and ameliorated cisplatin-induced nephrotoxicity through its antioxidant effects. Therefore, co-administration of pomegranate flower extract with cisplatin might be beneficial for these patients.

Please cite this paper as: Motamedi F, Nematbakhsh M, Monajemi R, Pezeshki Z, Talebi A, Zolfaghari B, et al. Effect of pomegranate flower extract on cisplatin-induced nephrotoxicity in rats. J Nephropathol 2014; 3(4):133-138. DOI: 10.12860/jnp.2014.26

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Revised: 21 Jun 2014
First published online: 01 Oct 2014
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