J Nephropathol. 2019;8(1): e8.
doi: 10.15171/jnp.2019.08
  Abstract View: 450
  PDF Download: 380

Original Article

Does curcumin or metformin attenuate oxidative stress and diabetic nephropathy in rats?

Soheila Asadi 1, Mohammad Taghi Goodarzi 1, Jamshid Karimi 1, Mohammad Hashemnia 2, Iraj Khodadadi 1 *

1 Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2 Department of Pathobiology, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran


Background: Since the importance of oxidative stress in the development of diabetic nephropathy (DN) has previously been established, the therapeutic effects of various natural antioxidant agents or synthetic drugs have so far been investigated.

Objectives: The aim of this study was to investigate the beneficial effects of curcumin (a natural polyphenol) and metformin (a common therapeutic medicine for type 2 diabetes) on oxidative status in kidney of type 1 diabetic rats.

Materials and Methods: In this experimental study 60 male Wistar rats were divided into 10 groups. Type 1 diabetes was induced by streptozotocin. Rats received chow diet and treated with either normal saline in control (N) and diabetic control (D) groups or different doses of metformin (Met) (300 or 500 mg/kg body weight) or curcumin (Cur) (50 or 150 mg/kg body weight) in N+Met300, N+Met500, N+Cur50, N+Cur150, D+Met300, D+Met500, D+Cur50, and D+Cur150 groups. Urinary creatinine, urea, and protein were measured. Total antioxidant capacity (TAC), total oxidant status (TOS), malondialdehyde (MDA), and the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase were assessed in kidney tissues.

Results: Both metformin and curcumin showed significant effects on urinary creatinine, urea, and protein levels (P value for all was <0.001). Unlike metformin, curcumin completely restored TAC and TOS (P<0.001), and MDA (P=0.012) in kidney tissues and significantly recovered the activities of SOD (P= 0.003), GPx (P< 0.001), and catalase (P=0.011).

Conclusions: Curcumin was found more effective than metformin in attenuating oxidative status in DN.

Implication for health policy/practice/research/medical education:

It has been shown that antioxidant agents might be useful in attenuating oxidative stress induced by DN. The present study comparatively investigated antioxidant properties of metformin and curcumin in kidney tissue of diabetic rats. Our results showed that although both metformin and curcumin could improve traditional biomarkers associated with DN, curcumin was more effective than metformin in reduction of oxidative stress in treated groups. Therefore, curcumin exhibited advantages on metformin and can be considered as a candidate therapeutic agent with potential anti-oxidative properties.

Please cite this paper as: Asadi S, Goodarzi MT, Karimi J, Hashemnia M, Khodadadi I. Does curcumin or metformin attenuate oxidative stress and diabetic nephropathy in rats?. J Nephropathol. 2019;8(1):e08. Doi: 10.15171/jnp.2019.08.

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Submitted: 29 Jul 2018
Accepted: 14 Nov 2018
First published online: 03 Dec 2018
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