J Nephropathol. 2019;8(2): e16.
doi: 10.15171/jnp.2019.16
  Abstract View: 195
  PDF Download: 156

Original Article

Losartan and magnesium sulfate administration reduce gentamicin-induced nephrotoxicity in rat model

Fatemeh Kourkinejad Gharaei 1, Tahereh Safari 2 * , Abbass Ali Niazi 3, Meysam Zeynali Bujani 1

1 Student Research Committee, Zahedan University of Medical Sciences, Zahedan, Iran
2 Department of Physiology, Zahedan University of Medical Sciences, Zahedan, Iran
3 Department of Pathology, Zahedan University of Medical Sciences, Zahedan, Iran

Abstract

Background: Nephrotoxicity is the most known side effect of gentamicin. In addition, renin angiotensin system (RAS) plays an important role in the pathogenesis of renal injury and nephrotoxicity. Hypomagnesaemia is other complication of gentamicin. Previous studies reported that magnesium plays an important role in cell enzymatic functions, reducing lipid peroxidation.

Objectives: We investigated the role of losartan and magnesium sulfate (MgSO4 ) on gentamicin nephrotoxicity.

Materials and Methods: In this study, rats randomly assigned to five groups. The first group, received saline, the second group received gentamicin 80 mg/kg/d, intraperitoneally (ip), and the third group, received a regular dose of losartan, 10 mg/kg/d + gentamicin 80 mg/kg/d. The fourth group received MgSO4 , 80 mg/kg/d + gentamicin 80 mg/kg/d. The fifth group obtained a continuous dose of gentamicin 80 mg/kg/d + losartan 10 mg/kg/d + MgSO4 80 mg/kg/d simultaneously. Nine days after administration of drugs, blood samples were collected from the heart. The level of urea, creatinine (Cr), malondialdehyde (MDA) and nitrite were measured in the animal serum and homogenized kidney tissue.

Results: Gentamicin increased serum urea and Cr levels. The administration of losartan and MgSO4 lonely and combination of them, significantly reduced the levels of serum urea and Cr. Losartan alone and combination of losartan and MgSO4 compared with gentamicin, significantly decreased kidney MDA level too. Decrease of kidney nitrite level by gentamicin was compensated by the administration of losartan, MgSO4 alone or their combination. Additionally, losartan and MgSO4 alone and their combination together significantly reduced renal damage.

Conclusions: The results of this study indicated that administration of losartan and MgSO4 individually and their combination decreased kidney nephrotoxicity and improved renal function. This effect is probably related to the improvement of antioxidant status and renal blood flow.

Implication for health policy/practice/research/medical education:

In an experimental study we found that administration of losartan and magnesium sulfate individually and in their combination reduced kidney nephrotoxicity and improved renal function. This effect is probably related to the improvement of antioxidant status and renal blood flow.

Please cite this paper as: Kourkinejad Gharaei F, Safari T, Niazi AA, Zeynali Bujani M. Losartan and magnesium sulfate administration reduce gentamicin-induced nephrotoxicity in rat model. J Nephropathol. 2019;8(2):e16. DOI: 10.15171/ jnp.2019.16.

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Submitted: 14 Apr 2018
Accepted: 02 Aug 2018
First published online: 20 Aug 2018
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