J Nephropathol. 2017;6(3):204-209.
doi: 10.15171/jnp.2017.34
PMID: 28975102
PMCID: PMC5607984
Scopus id: 85019837528
  Abstract View: 1940
  PDF Download: 1426

Original Article

Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and biochemical study

Hassan Ahmadvand 1,2, Gholamreza Shahsavari 2 * , Majid Tavafi 3, Shahrokh Bagheri 1, Mohamad Reza Moradkhani 4, Reza Mohammadrezaei Kkorramabadi 5, Peyman Khosravi 5, Maryam Jafari 1, Khadije Zahabi 6, Reza Eftekhar 5, Maryam Soleimaninejad 7, Sanaz Moghadam 1

1 Razi Herbal Researches Center, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
3 Department of Anatomy, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
4 Department of Anesthesia, Madani Heart Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
5 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.
6 Department of Animal Biology, Payam Noor University, Isfahan, Iran
7 Department of Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
*Corresponding author: Gholamreza Shahsavari, Ph D., Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. Email: reza70sh@yahoo.com

Abstract

Background: Oleuropein is a potent antioxidant and free-radical scavenger with antiinflammatory
properties.


Objectives:
In the present study, we evaluated the protective effects of oleuropein on
myeloperoxidase (MPO) activity, nitrite, urea, creatinine and glomerulosclerosis in
alloxan-induced type 1 diabetic rats.

Materials and Methods: Thirty Sprague-Dawley male rats were randomly divided into 3
groups: group 1 as control; group 2 as untreated diabetic; and group 3 as treated with
oleuropein 15 mg/kg i.p daily. Diabetes was induced in the second and third groups by
subcutaneous alloxan injection. After 48 days, the animals were anaesthetized and then
the livers and kidneys were removed immediately and used fresh or kept frozen until MPO
activity analysis. Blood samples were also collected before sacrificing to measure nitrite,
urea, and creatinine. Kidney paraffin sections were prepared to estimate glomerular
volume, leukocyte infiltration, and glomerulosclerosis.

Results: Oleuropein significantly decreased leukocyte infiltration and glomerulosclerosis in
the treated group compared with the diabetic untreated group. Oleuropein significantly
decreased the levels of urea, nitrite, and creatinine in the treated group compared with the
diabetic untreated group. Moreover, oleuropein significantly decreased MPO activity in
the treated group compared with the diabetic untreated group.


Conclusions:
Oleuropein has antioxidative and antiatherogenic activities and exerts beneficial
effects on inflammation and kidney function test and decreases diabetic complication in
diabetic rats.

Implication for health policy/practice/research/medical education:

Our study shows oleuropein reduced the leukocyte infiltration and glomerulosclerosis and levels of nitrite, urea and creatinine
and myeloperoxidase activity in diabetic treated group. The authors hope, the results of the present study help to improve
kidney function and the complication in diabetic patients.

Please cite this paper as: Ahmadvand H, Shahsavari G, Tavafi M, Bagheri S, Moradkhani MR, Mohammadrezaei Kkorramabadi R,
et al. Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and
biochemical study. J Nephropathol. 2017;6(3):204-209. DOI: 10.15171/jnp.2017.34.

First name
 
Last name
 
Email address
 
Comments
 
Security code


Article Viewed: 1940

Your browser does not support the canvas element.


PDF Downloaded: 1426

Your browser does not support the canvas element.