J Nephropathol. 2019;8(2): e15.
doi: 10.15171/jnp.2019.15
  Abstract View: 392
  PDF Download: 321

Original Article

The complete genome sequence BK polyomavirus study in kidney transplanted patients 

Manoochehr Makvandi 1, Gholam Abbas Kaydani 1 * , Heshmatollah Shahbazian 2, Alireza Teimoori 1, Fakher Rahim 3, Sajjad Aslani 4

1 Department of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Golestan Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Health Research Institute, Thalassemia and Hemoglobinopathies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4 Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
*Corresponding author: Gholam Abbas Kaydani, Email: Email: kaydani56g@gmail.com

Abstract

Background: BK polyomavirus is a member of the Polyomaviridae. This virus has spread worldwide and up to 82% of the world populations are serologically positive. BK polyomavirus usually transmits through inhalation or fecal-oral way in childhood, as well as is likely to cause an asymptomatic disease. The virus can be reactivated in people who are immunocompromised.

Objectives: In this study we aimed to determine the complete genome sequence of BK polyomavirus in 5 patients receiving kidney transplantation in Golestan hospital, Ahvaz, south west of Iran.

Materials and Methods: The study was performed on urine and blood samples from kidney transplant ward, Golestan hospital, Ahvaz, Iran. To amplify the whole genome of BK polyomavirus AccuPower ProFi Taq PCR PreMix kit (Bioneer, South Korea) was used. Then, purified fragments were cloned into the vector. Sequences derived from the sequencing process were assembled by MEGA7 software. Data were analyzed using MEGA7 software and a phylogenetic tree was built based on the maximum likelihood ratio method.

Results: Overall, 40 urine samples (40%) and two plasma samples (12%) were positive for BK virus DNA. For the selected samples, W.G. Long PCR was performed and 5.1 kbp fragment was observed in all five samples. These fragments were cloned and sequenced. Genomic sequence analysis of the 5 strains studied showed 97.6% homology and all our study samples were of the same clade, which could be a reason for our patients to be infected with the same strain. Comparing the 5 isolates in our study with reference strains, showed more than 98% of homology, and variation was observed in less than 2% of nucleotides.

Conclusion: These five isolates showed more than 98% homology compared to the reference sequences, which can be attributed to the fact that these patients are infected with a common strain or that the genomic stability in the strain exists in our geographic area. We can conclude that in our geographic region there is a genomic stability and this strain can also be used as a positive control sample in other parts of the country

Implication for health policy/practice/research/medical education:

The event of BK virus nephropathy practically in kidney transplant recipients shows that a merging of factors present around local injury and immunosuppression may lead to several complications, adverse events, and ultimately decreasing graft survival. Complete-genome sequencing leads to the availability of genomic information that can be used as a key to understanding the causes of health and disease. This is not a simple or rapid duty and will need considerable investment and personal, institutional, and governmental support; but with time and efforts, this technique can undoubtedly help in rapid examination of the etiology for complex diseases.

Please cite this paper as: Makvandi M, Kaydani GA, Shahbazian H, Teimoori A, Rahim F, Aslani S. The complete genome sequence BK polyomavirus study in kidney transplanted patients. J Nephropathol. 2019;8(2):e15. DOI: 10.15171/jnp.2019.15.

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Submitted: 18 May 2018
Accepted: 23 Sep 2018
First published online: 26 Oct 2018
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