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J Nephropathol. 2020;9(3): e26.
doi: 10.34172/jnp.2020.26

Scopus ID: 85084986107
  Abstract View: 418
  PDF Download: 118

Original Article

Effect of dimethyl sulfoxide in combat with gentamicin induced nephrotoxicity in rats

Afshin Hasanvand 1 ORCID logo, Majid Tavafi 2 * ORCID logo, Hassan Ahmadvand 3, Ahmad Tamjidipoor 2

1 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Department of Anatomy, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
3 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
*Corresponding author: Prof. Majid Tavafi, Ph.D, Email: mtavafi@yahoo.com and tavafi.m@lums.ac.ir

Abstract

Introduction: Gentamicin sulphate (GS) induces nephrotoxicity by increasing of reactive oxygen species (ROS).

Objectives: The aim of this research was to assess the renoprotective effect of dimethyl sulfoxide (DMSO) as an antioxidant agent against GS-induced nephrotoxicity.

Materials and Methods: Forty male rats (Sprague-Dawley) were divided equally in to five groups and were treated as follows; group 1; received normal saline and served as normal controls, group 2; received GS (100 mg/kg/d), groups 3; received GS and DMSO (0.5 mL/kg/d), groups 4; received GS and DMSO (1 mL/kg/d), groups 5; received GS and DMSO (2 mL/kg/d). After eight days of treatment, serum was prepared. Paraffin sections (3 µ thickness) were prepared from the left kidneys and stained through periodic acid-Schiff (PAS) method. Serum creatinine and urea were assessed by the kits and serum malondialdehyde (MDA) was assessed by thiobarbituric acid (TBA) test. Volume density of proximal tubules, tubular necrosis, tubular cast and lymphocyte infiltration were evaluated histopathologically. The data were analyzed by Mann-Whitney U test at P< 0.05 by SPSS 12 software.

Results: Serum MDA, creatinine, urea, tubular volume density, tubular necrosis, tubular cast and lymphocyte infiltration were ameliorated significantly in groups four and five compared with group two (P<0.05).

Conclusion: DMSO improved GS-induced renal toxicity significantly through prevention of lipid peroxidation and inflammation, but could not save kidney functional tests and histological changes at the same level as that of the normal group.

Keywords: Nephrotoxicity, Gentamicin, Dimethyl sulfoxide, Antioxidant, Reactive oxygen species, Renal toxicity

Implication for health policy/practice/research/medical education:

Nephrotoxicity due to gentamicin sulphate (GS) restricts its administration. Even though pathogenesis of GS-induced nephrotoxicity has not been understood entirely, recent investigations revealed that reactive oxygen species (ROS) participate in GS-induced nephrotoxicity. In this study dimethyl sulfoxide an antioxidant was used to inhibit GS-induced nephrotoxicity.

Please cite this paper as: Hasanvand A, Tavafi M, Ahmadvand H, Tamjidipoor A. Effect of dimethyl sulfoxide in combat with gentamicin induced nephrotoxicity in rats. J Nephropathol. 2020;9(3):e26. DOI: 10.34172/jnp.2020.26.

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Submitted: 09 Jun 2019
Accepted: 10 Aug 2019
ePublished: 10 Sep 2019
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