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J Nephropathol. 2017;6(1): 1-4.
doi: 10.15171/jnp.2017.01
PMID: 28042546
PMCID: PMC5106876
Scopus ID: 84992415374
  Abstract View: 3752
  PDF Download: 1712

Original Article

Tempol effect on epithelial-mesenchymal transition induced by hyperglycemia

Mohammad Jafari 1, Farahnaz Dadras 2, Hamid Reza Ghadimipour 1, Mohamad Ali Seif Rabiei 3, Farhad Khoshjou 4*

1 Pathology ward, Hamadan University of Medical Sciences, Hamadan, Iran
2 Nephrology Ward, Department of Internal Medicine, Iran University of medical Sciences, Tehran, Iran
3 Department of Community Medicine, Hamdan University of Medical Sciences, Hamadan, Iran
4 Urology and Nephrology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
*Corresponding Author: *Corresponding author: Farhad Khoshjou, Urology and Nephrology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran,, Email: fakhoshjoo@yahoo.com

Abstract

Background: One of common mechanisms in pathophysiology of chronic kidney diseases is epithelial-mesenchymal transition (EMT). On the other hand oxidative stress has been known to participate in kidney damage of diabetic nephropathy (DN).

Objectives: We studied if tempol, a well-known antioxidant agent, can ameliorate EMT in DN induced in male rats.

Materials and Methods: Twenty-seven male rats were equally divided in to 4 groups. Group I (control or C), group II (diabetic or D), group III (T) rats which was given tempol (100 mg/kg/day) by gavages for 28 days and group IV (D&T) was diabetic rats that also received same dose of tempol. After treatment, their kidneys were studied by immunohistochemicalstaining.

Results: Pathological changes in the kidney were detected concurrently with increasing kidney weight and urinary albumin excretion. In addition, EMT indices, i.e. decline of E-cadherin and increase of α SMA staining were significantly emerged in the renal tubular cells of diabetic group and were partially modified in diabetic group which were simultaneously treated by tempol.

Conclusions: Tempol can modify, but not significantly, EMT induced by DN.


Implication for health policy/practice/research/medical education:

Diabetic nephropathy (DN) is the major etiology of chronic kidney disease. On the other hand, oxidative stress has been well known to be involved in its pathophysiology. Besides, epithelial- mesenchymal transition (EMT) is a common finding in kidney injury. In current study, we tried to figure out if administration of tempol, an antioxidant agent, can modify EMT in DN.

Please cite this paper as: Jafari M, Dadras F, Ghadimipour HR, Seif Rabiei MA, Khoshjou F. Tempol effect on epithelialmesenchymal transition induced by hyperglycemia. J Nephropathol. 2017;6(1):1-4. DOI: 10.15171/jnp.2017.01.

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ePublished: 03 Aug 2016
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