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J Nephropathol. 2021;10(3): e31.
doi: 10.34172/jnp.2021.31

Scopus ID: 85108677829
  Abstract View: 1634
  PDF Download: 603

Original Article

Molecular patterns of oxidative stress in drug-induced nephropathy

Valentina Vitalyevna Lee 1* ORCID logo, Larissa Evgenievna Muravlyova 2 ORCID logo, Ryszhan Yemelevna Bakirova 1 ORCID logo, Ahmet Kiziltunc 3 ORCID logo, Zhanara Zholtaevna Turkhanova 1, Bolatkyl Dguzbaevna Ashirbekova 1 ORCID logo

1 Department of Internal Diseases, Karaganda Medical University, Karaganda-City, Kazakhstan
2 Department of Fundamental Medicine, Karaganda Medical University, Karaganda-City, Kazakhstan
3 Biochemistry Department, School of Medicine, Ataturk University, Erzurum, Turkey
*Corresponding Author: *Corresponding author: Valentina Vitalyevna Lee, Email: , Email: liv@qmu.kz

Abstract

Introduction: Drug induced kidney disorder is a frequent adverse event which contributes to morbidity and even incapacitation. The discovery and development of novel biomarkers and local (renal) response mechanisms, are needed for effective prevention of drug-induced nephrotoxicity.

Objectives: The main purpose of our study was to investigate the oxidative modifications of proteins in blood plasma and erythrocytes of patients with drug-induced nephropathies.

Patients and Methods: Around105 patients were divided into two groups: first group was represented by patients with psychotropic drug-induced nephropathy; the second one consisted of patients received nonsteroidal anti-inflammatory drugs (NSAIDs). Advanced oxidation protein products (AOPPs) were measured by Witko-Sarsat method. Protein reactive carbonyl derivatives (PRCD) were assayed in blood plasma and erythrocytes by the Levine method. Neutrophil gelatinaseassociated lipocalin (NGAL) was determined with the use of a commercially available ELISA kit.

Results: Carbonyl derivatives are significantly higher in red blood cells of the 1st and 2nd group patients compared to the control subjects. AOPP statistically increased both in patients with various types of drug nephropathy and in patients with chronic kidney disease (CKD) compared with the control group. The NGAL was significantly higher in all groups compared to the control subjects.

Conclusion: The patients with drug-induced nephropathy have increased level of oxidative stress products and response NGAL reaction. The mechanisms that lead to the development of oxidative stress and the production of modified proteins are different in patients treated with different drugs. Establishing patterns of cell-molecular interaction permit the drug-induced nephropathy to be timely diagnosed and therapeutic programs to be optimized.


Implication for health policy/practice/research/medical education:

Establishment of predictors of nephropathy progression in the context of their relationship with the dynamics of molecular-cell patterns will make it possible to formulate recommendations for the timely diagnosis of drug-induced nephropathies.

Please cite this paper as: Lee VV, Muravlyova LE, Bakirova RY, Kiziltunc A, Turkhanova ZZ, Ashirbekova BD. Molecular patterns of oxidative stress in drug-induced nephropathy. J Nephropathol. 2021;10(3):e31. DOI: 10.34172/jnp.2021.31.

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Submitted: 12 Feb 2020
Accepted: 04 Oct 2020
ePublished: 02 Nov 2020
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