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J Nephropathol. 2021;10(4): e41.
doi: 10.34172/jnp.2021.41
  Abstract View: 167
  PDF Download: 74

Original Article

A survey on lymphocyte T CD3, CD4 and CD8 in peripheral blood of kidney transplant recipients using mycophenolic acid

Thi Mai Dung Do 1,2 ORCID logo, Phan Hai An Ha 3, Van Dong Le 4, Quang Thuan Huynh 5 * ORCID logo

1 Department of Biochemistry, Viet Duc Hospital, Vietnam
2 Postgraduate in Department of Immunology, Military Medical University, Vietnam
3 Department of Kidney diseases and Dialysis, Viet Duc Hospital, Vietnam
4 Center for Training, Research Toxicology and Radioactivity, Military Medical University, Vietnam
5 Department of Biochemistry, Military Hospital 103, Military Medical University, Vietnam

Abstract

Introduction: Immunological monitoring could indirectly measure the suppressive effects of the drugs and provide early guidance on necessary preventive interventions in transplant recipients.

Objectives: Our goal was to determine whether mycophenolic acid (MPA) modulates peripheral blood lymphocyte T in kidney transplant recipients.

Patients and Methods: We assessed T lymphocytes CD3, CD4 and CD8 in peripheral blood in 30 donors and 35 recipients one day before and 10 days after transplantation using Becton Dickinson’s direct immune fluorescent light.

Results: Comparisons showed that the number of T lymphocytes CD3+, CD4+, CD8+ in peripheral blood of transplant recipients were lower than donors (TCD3 was 1690.31±503.45 versus 2280.73± 522.48; TCD4 was 549.51 ±211.72 cell/µL versus 766.37± 341.72 cell/µL and CD8 was 1134.37 ±431.07 cell/µL versus 1523.4± 349.23 cell/µL with P<0.001; P=0.001 and P= 0.0002 respectively). Additionally, post-transplantation lymphocytes TCD4 decreased in 10/35 of recipients and increased in 22/35 of recipients (P=0.036).

Conclusion: The T lymphocytes CD3, CD4 and CD8 in peripheral blood should be monitored at multiple post-transplant times to make early predictions of transplant rejection during follow-up treatment.

Keywords: T lymphocytes, Micophenolic Acid, Kidney transplant

Implication for health policy/practice/research/medical education: To determine whether mycophenolic acid (MPA) modulates peripheral blood lymphocyte T in kidney transplant recipients, we conducted a survey on lymphocyte T CD3, CD4 and CD8 in peripheral blood of 35 kidney transplanted recipients using mycophenolic acid. We assessed T lymphocytes CD3, CD4, CD8 in peripheral blood in 30 donors and 35 recipients one day before and 10 days after transplantation using Becton Dickinson’s direct immune fluorescent light. The results showed that the T lymphocytes CD3, CD4 and CD8 in peripheral blood should be monitored at multiple post-transplant times to make early prediction of transplant rejection during follow-up treatment.

Please cite this paper as: Dung Do TM, An Ha PH, Le V, Huynh Q. A survey on lymphocyte T CD3, CD4 and CD8 in peripheral blood of ِ kidney transplant recipients using mycophenolic acid. J Nephropathol. 2021;10(4):e41. DOI: 10.34172/jnp.2021.41.

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Abstract View: 167

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Submitted: 17 Jan 2021
Accepted: 28 Feb 2021
ePublished: 10 Apr 2021
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