Abstract
Background: Advanced glycation end-products (AGEs) cause proinflammatory responses and
macromolecular damages. Advanced oxidation protein products (AOPPs) are protein biomarkers
for oxidative stress. Levels of AGEs and AOPPs increase with the progression of chronic renal
dysfunction.
Objectives: In this study, we aimed to measure these species in patients with renal transplantation
and to analyze their correlation with the measured glomerular filtration rate (GFR) and renal
function parameters.
Patients and Methods: Eighty renal transplant patients and normal subjects were recruited.
GFR was measured by the two-sample plasma method with technetium-99m-labeled
diethylenetriaminepentaacetic acid (TC99m-DTPA) clearance. Biochemical measurements included
creatinine, cystatin C, urea, total protein, and pentosidine. Serum AGEs were determined using a
fluorometric assay and AOPPs were estimated spectrophotometrically.
Results: The measured GFR found to be significantly decreased in renal transplant patients compared
to the control subjects (P< 0.001). Levels of AGEs, AOPPs, serum creatinine, and cystatin C were
increased in renal transplant patients with lower values of measured GFR (mGFR). A significant
association between the levels of AGEs species (serum fluorescence and pentosidine) and mGFR
when adjusted for creatinine and other risk factors in multiple linear regression model analysis was
found (P=0.05 and P=0.001, respectively).
Conclusions: This study demonstrated increased levels of pentosidine and AGEs in transplant
recipients were associated with decreased mGFR. Their accumulation can be predictive for the
progression of chronic allograft loss of function.