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J Nephropathol. Inpress.
doi: 10.34172/jnp.2025.27684
  Abstract View: 28

Original Article

Stage-wise comparison of demographic characteristics and serum biochemical parameters in chronic kidney disease; a cross-sectional study

Abdullah Abbas Hamzah Al-Rubaye* ORCID logo

1 Department of Medical Lab Technology, College of Health and Medical Technology, Southern Technical University, Basra, Iraq
*Corresponding Author: Abdullah Abbas Hamzah Al-Rubaye, Email: abdulla.abbas@stu.edu.iq

Abstract

Introduction: Chronic kidney disease (CKD) is a progressive condition marked by declining glomerular filtration and disturbances in biochemical and electrolyte profiles; identifying stage‑specific changes in demographics and serum markers may improve early detection and guide stage‑appropriate management.

Objectives: This study compared demographic characteristics and serum biochemical parameters across stages 1–3 in patients with CKD to identify stage‑related differences associated with disease progression.

Patients and Methods: This cross‑sectional study enrolled 75 patients with early CKD (stage 1: n=24, stage 2: n=25, stage 3: n=26) attending specialist clinics at Al‑Fayhaa teaching hospital, Basra, Iraq (Feb–May 2025). Demographic data (age, sex) and fasting venous blood samples were collected for routine renal tests (creatinine, urea, estimated glomerular filtration rate [eGFR]), uric acid, total protein, albumin, total cholesterol, electrolytes (sodium [Na], potassium [K], chloride [Cl], magnesium [Mg], phosphate), and biomarkers such as neutrophil gelatinase-associated lipocalin (NGAL) and Cathepsin D. Group comparisons across CKD stages plus correlation analyses were performed to assess associations with disease progression.

Results: The results indicated that CKD progression was associated with male gender and older age, across with changes in serum electrolytes, kidney function tests, and biochemical parameters, including increasing creatinine, urea, uric acid, sodium, K, cholesterol, NGAL, and cathepsin D. The CKD progression also decreased eGFR, Mg, and total protein (P < 0.05), with no significant impact on albumin, phosphate, and Cl (P > 0.05).

Conclusion: CKD progression is associated with male gender, older age, and biochemical changes, including increased renal markers, lipids, sodium, K, NGAL, and cathepsin D, as well as decreased eGFR, Mg, and total protein. Albumin, phosphate, and Cl remained stable, demonstrating the complexity of CKD and the potential of new biomarkers.



Implication for health policy/practice/research/medical education:

In this study we found that chronic kidney disease (CKD) progression is closely linked to demographic factors such as male gender and older age, and is characterized by significant biochemical disturbances, including elevations in conventional renal markers, electrolytes, lipids, and emerging biomarkers like neutrophil gelatinase-associated lipocalin (NGAL) and cathepsin D, alongside reductions in estimated glomerular filtration rate (eGFR), magnesium, and total protein, while some parameters such as albumin, phosphate, and chloride remain unaffected, highlighting both the complexity of CKD-related metabolic changes and the potential value of novel biomarkers in disease monitoring.

Please cite this paper as: Al-Rubaye AAH. Stage-wise comparison of demographic characteristics and serum biochemical parameters in chronic kidney disease; a cross-sectional study. J Nephropathol. 2025;x(x):e27684. DOI: 10.34172/jnp.2025.27684.

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Submitted: 22 Sep 2025
Revision: 06 Dec 2025
Accepted: 07 Dec 2025
ePublished: 18 Dec 2025
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