Logo-jnp
J Nephropathol. 2017;6(3): 138-143.
doi: 10.15171/jnp.2017.24
PMID: 28975093
PMCID: PMC5607974
Scopus ID: 85019852587
  Abstract View: 3301
  PDF Download: 1889

Original Article

Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children

Mohammad Hashemi 1,2, Simin Sadeghi-Bojd 3,4*, Soheib Aryanezhad 4, Maryam Rezaei 2

1 Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
2 Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
3 Children and Adolescents Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
4 Department of Pediatrics, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
*Corresponding Author: *Corresponding author: Simin Sadegi-Bojd, Department of Pediatrics, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. , Email: sisadegh@yahoo.com

Abstract

Background: Primary nephrotic syndrome (NS) is a common kidney disease in children. Objectives: The present study was aimed to investigate whether rs5370 G>T (lys198Asn) genetic variant of endothelin-1 (ET-1) is involved in the susceptibility to NS.

Patients and Methods: This case-control study was performed on 138 patients with NS and 150 healthy children. Genomic DNA was extracted from whole blood using salting out method. Polymorphism of the ET-1 rs5370 G>T (lys198Asn) polymorphism detected by T-ARMS-PCR as well as PCR-RFLP method.

Results: The results showed that the genotype and allelic frequencies of the ET-1 rs5370 G>T variant were not significantly different between cases and controls. Furthermore, subgroup analysis showed that rs5370 G>T variant was not associated with gender of patients. In NS patients the genotype was not associated with cholesterol, triglyceride, total protein and albumin levels.

Conclusions: In conclusion, our findings indicate that ET-1 rs5370 G>T is not associated with NS. Further studies with larger sample sizes and different ethnicities are required to validate our findings.


Implication for health policy/practice/research/medical education:

In the present study we aimed to examine the impact of endothelin-1 (ET-1) rs5370 polymorphism on nephrotic syndrome (NS) in children. Our finding did not support an association between rs5370 variant and risk of NS in our population. In the forthcoming studies, other variants of endothelin-1 should be tested to find out the possible association between endothelin-1 polymorphisms and risk of NS.

Please cite this paper as: Hashemi M, Sadeghi-Bojd S, Aryanezhad S, Rezaei M. Association of endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children. J Nephropathol. 2017;6(3):138-143. DOI: 10.15171/jnp.2017.24.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 3302

Your browser does not support the canvas element.


PDF Download: 1889

Your browser does not support the canvas element.

ePublished: 17 Dec 2016
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)