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J Nephropathol. 2024;13(4): e21465.
doi: 10.34172/jnp.2023.21465
  Abstract View: 879
  PDF Download: 133

Original Article

Anti-oxidative and anti-inflammatory activity of Achatina fulica mucus in streptozocin-nicotinamide-induced diabetic kidney disease: an animal model study

Wachid Putranto 1,2* ORCID logo, Gigih Fitriawan 1,2 ORCID logo, Ratih Tri Kusuma Dewi 1,2 ORCID logo, Aryo Suseno 1,2 ORCID logo, Arief Nurudhin 1,3 ORCID logo, Yulyani Werdiningsih 1,3 ORCID logo, Santy Ayu Puspita Perdhana 1,2 ORCID logo, Nurhasan Agung Prabowo 1,4 ORCID logo, Yeremia Suryo Pratama 1,4 ORCID logo

1 Faculty of Medicine, Sebelas Maret University, Surakarta, Indonesia
2 Division of Nephrology, Department of Internal Medicine, Dr. Moewardi General Hospital, Surakarta, Indonesia
3 Division of Rheumatology, Department of Internal Medicine, Dr. Moewardi General Hospital, Surakarta, Indonesia
4 Department of Internal Medicine, Faculty of Medicine, Sebelas Maret University Hospital, Sukoharjo, Indonesia
*Corresponding Author: Wachid Putranto, Email: wachidputranto@staff.uns.ac.id

Abstract

Introduction: Diabetic kidney disease (DKD) progression resulted in increased intrarenal oxidative stress and increased inflammatory resulting in further renal fibrosis. Achatina fulica mucus was regarded to exerts anti-oxidative and anti-inflammatory effect.

Objectives: This study aims to observe the effect of administration of A. fulica mucus on oxidative stress and inflammation biomarkers in DKD-induced rats.

Methods and Materials: In this study, we used 32 males white Wistar rats divided into four groups; a control, and other three different groups induced with 45 mg/kg streptozocin (STZ) and 110 mg/kg nicotinamide (NA) intra-peritoneally. Achatina fulica mucus was administered orally in the last groups; 3.5 mL/d (S1), and 7 mL/d (S2). Post-test measurement of inflammatory and oxidative biomarker was used to determine the outcome.

Results: The study resulted in reduction of malondialdehyde (MDA), transforming growth factor-β (TGF-β), tumor necrosis factor-α (TNF-α), high sensitivity C-reactive protein (hs-CRP), vascular endothelial growth factor (VEGF), and interleukin-1β (IL-1β) in A. fulica mucus administration in our STZ-NA induced rats, with higher dose of the mucus further reduce the inflammatory and oxidative stress biomarkers.

Conclusion: Current study showed the potential of A. fulica mucus usage in future management of inflammation and oxidative stress in diabetes and DKD.


Implication for health policy/practice/research/medical education:

Achatina fulica mucus has the potential to be developed as an adjuvant therapy for patients with diabetic kidney disease; however, additional clinical research is required.

Please cite this paper as: Putranto W, Fitriawan G, Dewi RTK, Suseno A, Nurudhin A, Werdiningsih Y, Perdhana SAP, Prabowo NA, Pratama YS. Anti-oxidative and anti-inflammatory activity of achatina fulica mucus in streptozocin-nicotinamide-induced diabetic kidney disease: an animal model study. J Nephropathol. 2024;13(4):e21465. DOI: 10.34172/jnp.2023.21465.

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Submitted: 16 Apr 2023
Accepted: 01 Jul 2023
ePublished: 23 Sep 2023
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