Parto Nasri
1 
, Niloufar Hooshyar
2 , Banafsheh Yalameha
3 , Mahsa Akafzadeh Savari
4 , Mohammadreza Khosravifarsani
5 , Saied Mardani
6 , Pegah Noorshargh
7 , Ali Hasanpour Dehkordi
8 , Hamid Nasri
4,9* , Elnaz Golestaneh
10 , Elham Bijad
11, Behzad Ghasemi
12 , Nafiseh Nowrouzi
12 1 Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
2 Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
4 Nickan Research Institute, Isfahan, Iran
5 Cancer Prevention Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
6 Department of Internal Medicine, Medicine Faculty, Shahrekord University of Medical Sciences, Shahrekord, Iran
7 Alzahra Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
8 Social Determinants of Health Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
9 Department of Internal Medicine, Isfahan, University of Medical Sciences, Isfahan, Iran
10 Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
11 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
12 Faculty of Pharmacy, University of Pécs, Pécs, Hungary
Abstract
Introduction: The administration of iodinated contrast medium is extensive in various imaging techniques including coronary angiography. Objectives: The present investigation was designed to examine the ameliorative effect of clove buds (Syzygium aromaticum) on contrast-induced acute kidney injury (CI-AKI) in rats
Materials and Methods: Forty male Wistar rats with a mean body weight of 200-250 g were studied. Rats randomly assigned into four groups, 10 rats for each; Group 1; normal rats (control group; sham group); they did not receive any drugs. Group 2; rats were received 10 mL/kg as a single dose of iodixanol (contrast media) by intravenous (IV) injection. Group 3; rats received 10 mg/kg clove by intraperitoneal (IP) injection for three days, while in day forth, they received a single dose of iodixanol (10 mL/kg). Group 4; rats of this group first received a single dose of iodixanol (10 mL/ kg). Then rats treated by clove (10 mg/kg) by intraperitoneal (IP) injection for three days (days 2, 3 and 4th). Kidney sections were examined for degeneration, flattening, and necrosis of renal tubular cells and also dilatation of tubular lumen.
Results: We found a significant difference between groups regarding the sum of injury (degeneration, flattening, necrosis and dilatation). A significant difference in types of injury (degeneration, flattening, necrosis and dilatation) among the groups (P=0.001) was seen too. We detected a significant difference between groups II (contrast media) and III (rats pretreated by clove; P<0.001). Accordingly, we detected a significant difference between groups II (contrast media) and IV (rats post-pone treated by Clove buds; P<0.001) too. There was not a significant difference between groups of III and IV (P> 0.05).
Conclusion: In this animal study, we found that post-pone treatment of clove was as effective as pretreatment to mitigate the injury induced by contrast media.