Parto Nasri
1 
, Pegah Noorshargh
2 , Niloufar Hooshyar
3 , Mahsa Akafzadeh Savari
4 , Banafsheh Yalameha
5 , Elnaz Golestaneh
6 , Mohammadreza Khosravifarsani
7 , Saied Mardani
8 , Ali Hasanpour Dehkordi
9 , Azar Baradaran
4,10,11* , Elham Bijad
12, Behzad Ghasemi
13 , Nafiseh Nowrouzi
13 1 Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
2 Alzahra Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
3 Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Nickan Research Institute, Isfahan, Iran
5 Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
6 Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
7 Cancer Prevention Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
8 Department of Internal Medicine, Medicine Faculty, Shahrekord University of Medical Sciences, Shahrekord, Iran
9 Social Determinants of Health Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
10 Inflammation and Tissue Response Research Center, Isfahan, Iran
11 Department of Pathology, Isfahan, University of Medical Sciences, Shahrekord, Iran
12 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
13 Faculty of Pharmacy, Pécs University, Pécs, Hungary
Abstract
Introduction: Contrast-induced nephropathy (CIN) is associated with a minimum increase of 25% of the creatinine base or an increase in creatinine of 0.5 mg/dL during 2-3 days after the administration of the contrast agent
Objectives: The present investigation was designed to examine the effect of sodium hydrogen sulfide (NaHS) on contrast-induced acute kidney injury in rats.
Materials and Methods: Forty male Wistar rats randomly assigned into four groups, 10 rats for each group. Group 1; normal rats (control group; sham group); they did not receive any drugs. Group 2; rats were received 10 mL/kg as a single dose of iodixanol (contrast media) by intravenous (IV) injection. Group 3; they received 10 mg/kg of NaHS by intraperitoneal (IP) injection for three days, while in day forth, rats received a single dose of iodixanol (10 mL/kg). Group 4; rats of this group, first received a single dose of iodixanol (10 mL/kg). Then rats were treated by NaHS (10 mg/kg) by intraperitoneal (IP) injection for 3 days (days 2, 3 and 4).The kidneys were removed immediately after sacrificing and prepared for morphological examination. Kidney sections were examined for intensity of kidney damage by examination for degeneration, flattening, and necrosis of renal tubular cells and also dilatation of tubular lumen.
Results: We detected a significant difference between groups regarding the morphologic variables of damage (P=0.001; one-way ANOVA). A significant difference of morphologic variables of damage (degeneration, flattening, necrosis, and dilatation) among the groups was seen too (P=0.001; one-way ANOVA). The study showed a significant difference between groups II (contrast media) with III (rats pretreated by NaHS) (P<0.001). Moreover, we detected a significant difference between groups II (contrast media) and IV (rats post-pone treated by NaHS) (P<0.001). However, there was not a significant difference between the groups of III and IV (P>0.05).
Conclusion: Post-pone treatment of NaHS was as effective as the pretreatment to mitigate the renal damage induced by contrast media.